Transient Transient Transfection Factors for Expression of Recombinant Somatostatin Plasmid pEGS/2SS in Cultured HeLa Cells

We aimed to investigate the factors infecting transfection efficiency of somatostatin recombinant plasmid pEGS/2SS introducting into HeLa cells,make preparations for advanced study on somatostatin gene vaccine mechanism and effect of action.Four parameters including transfection reagent(lipofectamineTM) concentration,DNA concentration,cells cultured time after transfection and DNA extracting techniques were analyzed and optimized,and transfection efficiency was evaluated by the percentage of transfected cells with green fluorescence under fluorescence microscope.Based on the optimized lipofectamineTM and DNA concentration,34.02% transfected HeLa cells could express green fluorescence 72 h after transfection when the concentration of lipofectamineTM and DNA was individually 6 μg and 1 μg;Based on the optimized cells cultured time after transfection and DNA extracting techniques,17.88% transfected HeLa cells could express green fluorescence protein with Plasmid Maxi Kit extracting DNA 48 h after trasfection.Optimal transfection conditions were determined with four parameters,17.88% HeLa cells could express green fluorescence protein with Plasmid Maxi Kit extracting DNA,1 μg DNA and 6 μg lipofectamineTM 48 h after transfection.