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Comparative Study of Antioxidant Activity of Different Solvent Extracts of Ganoderma lucidum

Xie Ming-yong

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Abstract

Dried Ganoderma lucidum was sequentially extracted with petroleum ether, chloroform, methanol and water and the methanol extract was fractionated into ethyl acetate- and n-butyl alcohol-soluble fractions. The antioxidant abilities of the extracts from Ganoderma lucidum obtained were evaluated by DPPH radical scavenging, Folin-Ciocalteu and FRAP assays. All the extracts exhibited strong antioxidant abilities in a dose-dependent manner. These extracts from Ganoderma lucidum had a synergistic effect in the DPPH scavenging assay. The ethyl acetate-soluble fraction had the strongest antioxidant activity, followed by the water extract.

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What this paper is about

Dried Ganoderma lucidum was sequentially extracted with petroleum ether, chloroform, methanol and water and the methanol extract was fractionated into ethyl acetate- and n-butyl alcohol-soluble fractions. The antioxidant abilities of the extracts from Ganoderma lucidum obtained were evaluated by DPPH radical scavenging, Folin-Ciocalteu and FRAP assays. All the extracts exhibited strong antioxidant abilities in a dose-dependent manner. These extracts from Ganoderma lucidum had a synergistic effect in the DPPH scavenging assay. The ethyl acetate-soluble fraction had the strongest antioxidant activity, followed by the water extract.

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Available abstract

Dried Ganoderma lucidum was sequentially extracted with petroleum ether, chloroform, methanol and water and the methanol extract was fractionated into ethyl acetate- and n-butyl alcohol-soluble fractions. The antioxidant abilities of the extracts from Ganoderma lucidum obtained were evaluated by DPPH radical scavenging, Folin-Ciocalteu and FRAP assays. All the extracts exhibited strong antioxidant abilities in a dose-dependent manner. These extracts from Ganoderma lucidum had a synergistic effect in the DPPH scavenging assay. The ethyl acetate-soluble fraction had the strongest antioxidant activity, followed by the water extract.

Key concepts: Ethyl acetate, Petroleum ether, Chemistry, Antioxidant, DPPH, Chloroform, Ganoderma, Methanol

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