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2001•Medical Journal of National Defending Forces in Northwest ChinaRequires access

Stellera chamaejasme L. drug-serum of mouse induced apoptosis of K562 cells

Jun Fan

Open publisher page 1 citations

Abstract

Objective:To explore the antitumor mechanism of Stellera chamaejasme L. (SCL) on apoptosis.Methods:SCL drug-serum was derived from mice after treatment with different doses of SCL.Cultured human leukemia K562 cells were incubated with the drug-serum.Inhibition of proliferation was measured using MTT assay.Morphological assessment of apoptosis was performed with fluorescence microscope.DNA fragmentation was assessed by flow cytometry.Results:Exposure of exponentially growing K562 cells to SCL drug-serum of mouse (treated with SCL 5, 10, and 20 g·kg -1 ) for 48 h resulted in growth inhibition in a dose-dependent manner.Typical apoptosis morphological changes and DNA fragmentation in K562 cells were induced.Apo bodies in the cells were observed, and the percentage of apoptotic cells with fractional DNA content increased from 11.7% to 58.2%.Conclusion: SCL could induce apoptosis of K562 cells.

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What this paper is about

Objective:To explore the antitumor mechanism of Stellera chamaejasme L. (SCL) on apoptosis.Methods:SCL drug-serum was derived from mice after treatment with different doses of SCL.Cultured human leukemia K562 cells were incubated with the drug-serum.Inhibition of proliferation was measured using MTT assay.Morphological assessment of apoptosis was performed with fluorescence microscope.DNA fragmentation was assessed by flow cytometry.Results:Exposure of exponentially growing K562 cells to SCL drug-serum of mouse (treated with SCL 5, 10, and 20 g·kg -1 ) for 48 h resulted in growth inhibition in a dose-dependent manner.Typical apoptosis morphological changes and DNA fragmentation in K562 cells were induced.Apo bodies in the cells were observed, and the percentage of apoptotic cells with fractional DNA content increased from 11.7% to 58.2%.Conclusion: SCL could induce apoptosis of K562 cells.

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Available abstract

Objective:To explore the antitumor mechanism of Stellera chamaejasme L. (SCL) on apoptosis.Methods:SCL drug-serum was derived from mice after treatment with different doses of SCL.Cultured human leukemia K562 cells were incubated with the drug-serum.Inhibition of proliferation was measured using MTT assay.Morphological assessment of apoptosis was performed with fluorescence microscope.DNA fragmentation was assessed by flow cytometry.Results:Exposure of exponentially growing K562 cells to SCL drug-serum of mouse (treated with SCL 5, 10, and 20 g·kg -1 ) for 48 h resulted in growth inhibition in a dose-dependent manner.Typical apoptosis morphological changes and DNA fragmentation in K562 cells were induced.Apo bodies in the cells were observed, and the percentage of apoptotic cells with fractional DNA content increased from 11.7% to 58.2%.Conclusion: SCL could induce apoptosis of K562 cells.

Key concepts: Apoptosis, K562 cells, Flow cytometry, DNA fragmentation, Molecular biology, Fragmentation (computing), Leukemia, Fluorescence microscope

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