2013•Hebei yiyaoRequires access
Effect of valsartan combined with benazepril on the expression of nephrin protein in rats with diabetic nephropathy
Zhang Xiuxian
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Abstract
Objective To observe the effect of valsartan combined with benazepril on the expression of nephrin protein in rats with diabetic nephropathy.Methods The male SD rats were randomly divided into normal control group(NC group)and experimental group.The animal models with diabetic nephropathy were established in experimental group by unilateral nephrectomy + STZ intraperitoneal injection,then the rat models were randomly divided into diabetic nephropathy group(DN group)and combined treatment group(CT group).After 8 weeks the expression indexes of nephrin in kidney tissue were detected by immunohistochemistry,at the same time,the pathological changes of renal glomerulus podocyte were observed by electron microscope.Results As compared with that in NC group,the expression of nephrin in DN group was significantly down-regulated(P0.01),so was in CT group(P0.05),furthermore,there was a significant difference between CT group and DN group(P0.01).The changes of nephrin showed by immunohistochemisty and pathological changes of renal glomerulus podocyte in NC group were similar to those in CT group,however,which in DN group were more obvious.Conclusion The combined application of valsartan with benazepril can enhance the expression of nephrin,promote the repair of podocytes so as to retard the progress of diabetic nephropathy.