Construction of PPARγ transactivation model with luciferase reporter gene

PPARγ is a nuclear transcription factor which involves in many major diseases. It is not only a hot spot of basic research but also a critical target of drugs screening in present years. In this study, a recombinant plasmid expressing a complete PPARγ1 protein was transfected into human Jarket cells. After the expression of PPARγ1 protein was confirmed by RT-PCR , the recombinant plasmid was co-transfected in Jarket cells instantly with another plasmid pTK-PPRE which contains PPARγ response element and a luciferase reporter gene. The luciferase expression in the co-transfected cell was detected out and raised sharply with stimulation of 15d-PGJ2, a strong agonist of PPARγ. The experimental result indicated that the PPARγtransactivation model was successfully constructed. This model would be useful in development of PPARγ ligands and other relative research.