Effects of Combination of Arsenic Trioxide and Oxaliplantin on Viability of Hepatocellular Carcinoma Cell Line HepG2 in Vitro

Objective To explore the inhibiting effect of Arsenic trioxide(As2O3)combined with Oxaliplantin(L-OHP) on hepatocellular carcinoma cell line HepG2.Methods The inhibiting effect on the cells growth was observed by MTT assay.The character of the two drugs interaction was estimated by coefficient of drug in interaction(CDI).The cell cycle and apoptotic changes were studied by the flow cytometry(FCM).Results The inhibiting and apoptosis inducing effect were significantly higher by the combination of As2O3 and L-OHP than by any drug used alone.The cell cycle of HepG2 cells was arrested in G2/M phase by As2O3,in S+G2/M by L-OHP,and in S+G2/M phase by As2O3 combined with L-OHP.Conclusion As2O3 combined with L-OHP in moderate-low concentration have synergistic effect in inhibiting the cell growth of HepG2 cell line possibly by inducing carcinoma cell apoptosis and enhancing the function of cell cycle arrest.