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HPLC determination of the content and entrapment efficiency of artemisia ketone in compound tretinoin liposome

Feng Wen-yu

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Abstract

Objective:To establish a HPLC method for the content and entrapment efficiency determination of artemisia ketone in compound tretinoin liposome.Methods:The separation was carried out on a Dikma Kromasil C18 column(250 mm×4.6mm,5 μm).The mobile phase was acetonitrile-water(75∶25).The detective wavelength was set at 242 nm.The column temperature was 30℃.The flow rate was 0.8 ml/min and the sample size was 10 μl.Results:The calibration curve showed good linearity over the range of 25.16～251.60 μg/ml(r=0.999 9).The average recovery was 99.53%with RSD of 0.72%.Conclusion:This method is simple,rapid,accurate and with good repeatability and recovery.It can be used as a quantitative analysis method for this preparation.

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What this paper is about

Objective:To establish a HPLC method for the content and entrapment efficiency determination of artemisia ketone in compound tretinoin liposome.Methods:The separation was carried out on a Dikma Kromasil C18 column(250 mm×4.6mm,5 μm).The mobile phase was acetonitrile-water(75∶25).The detective wavelength was set at 242 nm.The column temperature was 30℃.The flow rate was 0.8 ml/min and the sample size was 10 μl.Results:The calibration curve showed good linearity over the range of 25.16～251.60 μg/ml(r=0.999 9).The average recovery was 99.53%with RSD of 0.72%.Conclusion:This method is simple,rapid,accurate and with good repeatability and recovery.It can be used as a quantitative analysis method for this preparation.

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Available abstract

Objective:To establish a HPLC method for the content and entrapment efficiency determination of artemisia ketone in compound tretinoin liposome.Methods:The separation was carried out on a Dikma Kromasil C18 column(250 mm×4.6mm,5 μm).The mobile phase was acetonitrile-water(75∶25).The detective wavelength was set at 242 nm.The column temperature was 30℃.The flow rate was 0.8 ml/min and the sample size was 10 μl.Results:The calibration curve showed good linearity over the range of 25.16～251.60 μg/ml(r=0.999 9).The average recovery was 99.53%with RSD of 0.72%.Conclusion:This method is simple,rapid,accurate and with good repeatability and recovery.It can be used as a quantitative analysis method for this preparation.

Key concepts: Chromatography, Repeatability, High-performance liquid chromatography, Calibration curve, Chemistry, Content determination, Liposome, Detection limit

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HPLC determination of the content and entrapment efficiency of artemisia ketone in compound tretinoin liposome — Research Paper | ScholarLens