Cloning of hsa-miR-106b and construction of its lentiviral expression vector

Aim To construct a lentiviral expression vector for hsa-miR-106b.Methods The pre-mir-106b amplified by PCR was inserted into pLVTHM.The recombinant plasmid pLVTHM-miR-106b was confirmed by restriction endonuclease analysis and DNA sequence.293FT cells were cotransfected with lentivirus vector pLVTHM-miR-106b,psPAX2 and pMD2.G.All virus stocks were produced by calcium phosphate-mediated transfection.Results Restriction enzyme digestion and DNA sequencing demonstrated that the lentivirus vector pLVTHM-miR-106b was constructed successfully.Conclusion The successfully construction of lentivirus vector pLVTHM-miR-106b provides the basis for the further study of molecular function of mir-106b.