Influence of proliferation and adhesion ability in MG63 cells induce by KiSS-1 gene

OBJECTIVE: To investigate the proliferation,adhesion ability in MG63 cells transfected by PSNAV2.0-KiSS-1.METHODS: KiSS-1 expression plasmid PSNAV2.0-KiSS-1 was constructed,and transfected into MG63 cells.MG63 cells stably transfected with PSNAV2.0-KiSS-1(named MG63-KiSS-1) were selected by G418.KiSS-1 mRNA and protein expression in MG63-KiSS-1 cells was examined by real-Time PCR and Western blotting,respectively.To examine the effect of the transfection in vitro by cell proliferation.To test the difference of adhesion ability between the transfected and non-transfected cell lines by use of tumor adhesion experiment.RESULTS: MG63 cells stably transfected with PSNAV2.0-KiSS-1 were successfully established.MG63-KiSS-1 cells over expressed KiSS-1 protein.The cell proliferation was observed to be slow down in MG63-KiSS-1 cells,especially 48 hours after transfection(F=10.05,P0.05).The adhesion ability of MG63 cells were also significantly inhibted after PSNAV2.0-KiSS-1 transfection caompared with vasa umbilicalis endothelial cells,FN and matrigel(P0.05).CONCLUSIONS: KiSS-1 can inhibit the cell proliferation and adhesion ability of MG63 cells significantly.And it may be play a key role in gene therapy of osteosarcoma.