Effect of DEK gene silencing on proliferation and apoptosis in human cervical cancer CaSki cell

OBJECTIVE:To study the impact and mechanism of DEK gene siRNA in human cervical cancer CaSki cell by observing of proliferation,cell cycle and apoptosis after DEK gene silencing in human cervical cancer CaSki cell after DEK gene silencing.METHODS: The DEKsiRNA eukaryotic expression vector into CaSki cell,cell proliferation examined by MTT assay,cell cycle and cell apoptosis were analyzed by flow cytometry at 48h post DEK siRNA transfection.RESULTS: The growth inhibitin rate(53.2%,P=0.038) and apoptosis rate((13.84±3.19)%,P=0.002)) of CaSki cells of psiRNA-hHDEK transfection increase significantly by comparing CaSki cells of non-transfected and psiRNA-hH1neo transfected;the cell number of G0/G1 phase in psiRNA-hHDEK transfection group(82.65±5.23) was higher than in controls(70.25±5.11) and in psiRNA-hH1neo transfection group(74.36±7.49),the difference was significant(P=0.003);proliferation index of psiRNA-hHDEK transfected cell was 17.35%,lower than non-transfected group 29.75% and psiRNA-hH1neo transfection 25.64%,the difference was significant(P=0.02).CONCLUSION: DEK gene silencing can inhibit reproduction and synthesis of DNA in tumor cells and inhibite cell proliferation and cell division,induce the apoptosis of CaSki cell.