Optimization of Electroporation Parameters for K562 Cells DNA transfection

Objective To optimize the electroporation parameters in K562 cell using GFP (green fluorescent protein) as the observation index.Methods The plasmid DNA was transferred into K562 cell by electroporation using differently experimental conditions including the plasmid density,the volume of cell suspension and the operation temperature.The transfection efficiency was evaluated by flow cytometry(FCM) and fluorescent microscopy.Results The highest transfec- tion rate (58.6% for K562 Cell) was achieved under the condition of voltage 250 V,electric capaci- ty 950μF.The higher transfection rate was achieved when the volume of cell suspension is 0.4 mL and the plasmid density is more than 20μg/mL.The operation temperature and the concentration of serum in buffer have no effect on the transfection efficiency.Conclusion Electroporation is a highly efficient gene transfection method.The transfection efficiency can be improved by optimizing electroporation parameters.