Effects of different culture conditions on mycelium growth of Agaricus bisporus

[Objective]The present experiment was conducted to investigate the biological characteristics of different Agaricus bisporus strains in order to breed good quality Agaricus bisporus varieties suitable for cultivation in Guangxi and to develop proper management measures.[Method]The morphological characteristics of seven Agaricus bisporus strains were investigated and effects of temperature,pH,illumination,carbon and nitrogen sources on their mycelial growth were studied.[Result]Under the similar cultivation conditions,the strain 9506 showed the highest mycelial growth rate,while it was the lowest in portobello mushroom.The appropriate temperature for mycelial growth of Agaricus bisporus ranged from 20-30℃ with the optimal temperature being 25℃.The suitable pH for its mycelial growth was 4.0-9.0 with the best pH range between 6.0-8.0.Illumination was found to slightly affect the mycelial growth.The optimal carbon and nitrogen sources were glucose and yeast powder,respectively.[Conclusion]No significant genetic differences were observed amongst seven Agaricus bisporus species.Temperature,pH,illumination,carbon and nitrogen sources should be taken into account during cultivation to attain high and steady yield.