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2010•Journal of Pathogen BiologyRequires access

Detection of the isoniazid-resistant katG gene of Mycobacterium tuberculosis.

Xue Xin, Yi Qiu, Yonglie Chu, Sihai Zhao, Mingming Shao, Chunxia Cao

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Abstract

Objective To study the molecular mechanism of Mycobacterium tuberculosis resistance to isoniazid (INH) and to establish a method of rapid detection of katG gene mutations in M.tuberculosis.Methods The katG genes of 105 M.tuberculosis clinical isolates were tested by PCR-RFLP and verified by DNA sequencing and bioinformatics analysis.Results Strain H37Rv was used as a control.In 105 M.tuberculosis clinical isolates,5 of 34 (14.7%) drug-susceptible isolates and 55 of 105 (52.4%) INH-resistant isolates had a katG gene mutation according to PCR-RFLP.Conclusion Drug resistance of M.tuberculosis to INH is related to katG gene mutation,PCR-RFLP might serve as a method of rapidly detecting the INH resistance of M.tuberculosis.

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What this paper is about

Objective To study the molecular mechanism of Mycobacterium tuberculosis resistance to isoniazid (INH) and to establish a method of rapid detection of katG gene mutations in M.tuberculosis.Methods The katG genes of 105 M.tuberculosis clinical isolates were tested by PCR-RFLP and verified by DNA sequencing and bioinformatics analysis.Results Strain H37Rv was used as a control.In 105 M.tuberculosis clinical isolates,5 of 34 (14.7%) drug-susceptible isolates and 55 of 105 (52.4%) INH-resistant isolates had a katG gene mutation according to PCR-RFLP.Conclusion Drug resistance of M.tuberculosis to INH is related to katG gene mutation,PCR-RFLP might serve as a method of rapidly detecting the INH resistance of M.tuberculosis.

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Available abstract

Objective To study the molecular mechanism of Mycobacterium tuberculosis resistance to isoniazid (INH) and to establish a method of rapid detection of katG gene mutations in M.tuberculosis.Methods The katG genes of 105 M.tuberculosis clinical isolates were tested by PCR-RFLP and verified by DNA sequencing and bioinformatics analysis.Results Strain H37Rv was used as a control.In 105 M.tuberculosis clinical isolates,5 of 34 (14.7%) drug-susceptible isolates and 55 of 105 (52.4%) INH-resistant isolates had a katG gene mutation according to PCR-RFLP.Conclusion Drug resistance of M.tuberculosis to INH is related to katG gene mutation,PCR-RFLP might serve as a method of rapidly detecting the INH resistance of M.tuberculosis.

Key concepts: Isoniazid, Mycobacterium tuberculosis, Tuberculosis, Restriction fragment length polymorphism, Biology, Drug resistance, Gene, Gene mutation

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Detection of the isoniazid-resistant katG gene of Mycobacterium tuberculosis. — Research Paper | ScholarLens