Optimization of fermentation condition of Glucose-1-phosphate production by Bacillus subtilis XH-13_UV3-8 with response surface methodology

Response surface methodology was used to optimize the fermentation condition of glucose-1-phosphate(G-1-P)production by Bacillus subtilis XH-13_UV3-8.Firstly,a Plackett-Burman design was used to evaluate the influence of 12 related factors.Yeast extract powder and K2HPO4·3H2O influenced G-1-P production positively while pH did negatively.The path of steepest ascent was used to approach the optimal region of the fermentation condition subsequently.Box-Behnken design was used to optimize the three critical factors mentioned above,the optimum levels and the relationships among these factors were found out by quadratic regression model equation with Design-Expert statistic methods,the optimal levels of the variables were determined as follows:yeast extract powder 0.8%,K2HPO4·3H2O 0.7% and pH 6.6.The optimized condition allowed the G-1-P production to increased from 6.85mg/mL to 8.56mg/mL.