The initial research of interleukin-17 and its receptor in the process of cerebral ischemia

Objective To discuss the expression and the damage to neurons of the action of IL-17 and IL-17R during the process of cerebral ischemic with the model of mouse permanent middle cerebral artery occlusion(pMCAO).Method The model of mouse permanent middle cerebral artery occlusion was made by introduction of an intraluminal nylon.The change of morphology of the brain tissue was observe,the expression of IL-17 in serum and ischemic cortical was detected by ELISA and immunohistochemistry,respectively.The expression of Th17 and IL-17R were detected by double-immunofluorescence.Neurons by primary culture were identified by immunofluorescence,and then detected the damage of IL-17 on neurons under the condition of Oxygen-Glucose Deprivation(OGD) with MTT.Results The level of IL-17 in serum revealed a gradually increased value beginning from 24h after pMCAO and significant differences at 24h,2d and 6d in comparison to control values(P24h0.05,P2d,6d0.001).There were Th17 and IL-17R expression in the ischemic tissue.Neurons with IL-17 cultured after OGD 2h had significant damage to neurons compared with pure neuron cultured after OGD 2h,P10ng/ml IL-170.05,P100ng/ml IL-170.01,and higher density of IL-17 had more serious damage to neurons.Conclusion IL-17 and IL-17R participate the process of cerebral ischemic.There is Th17 and IL-17R expression in ischemic brain.The effects of IL-17 which are dose dependent aggravate the damage to neurons in ischemic injury.