Analysis on microbial community structure in A~2/O process

For an analysis of microbial diversity in the A2/O process,the polymerase chain reaction(PCR) and denaturing gradient gel electrophoresis(DGGE) techniques were applied.The genome DNA extracted from the sludge was amplified with general primers 530F/1490R to 16s rRNA gene of bacteria and archaebacteria,after purification the PCR products were used for DGGE analysis.The results reveal that the microorganism communities of sludge in A2/O process are very abundant.There are 19 kinds of microorganisms at the aerobic region, 18 kinds at the anoxic region and 15 kinds at the anaerobic region;at different regions of the A2/O process there are some special species of genus and common species of genus,and the succession of microbial communities in the A2/O process is not obvious,The similarity of the microbial communities in the process is 71.40%,indicating that the community structure is fairly stable.