Tamoxifen stimulates the proliferation of cultured rat cervical muscle cells

Objective:To investigate whether tamoxifen is involved in the proliferation of the cultured rat cervical muscle cells and explore its possible mechanism.Methods:The level of cell proliferation was estimated by drawing proliferation curves of rat cervical muscle cells,assessing OD value by MTT assay,techniques of flow cytometry and laser scanning confocal microscope. Results: Tamoxifen (10 -6 mol·L -1 ) shifted the curve upward. Tamoxifen (10 -8 to 10 -6 mol·L -1 ) significantly stimulated the level of cell proliferation. Tamoxifen (10 -6 mol·L -1 ) stimulated G_1/S transfer of the cell cycle and increased the releasing of calcium obviously. Conclusion:It is suggested that tamoxifen modulates the proliferation of the cultured rat cervical muscle cells. Tamoxifen may stimulate the proliferation through modulating cell cycle and calcium in the cells.