Clone and Prokaryotic Expression of Non-Structure Protein 3AB & 3A Genes in Foot-and-Mouth Disease

Based on the construction of template of non-structure protein 3ABC and plasmid pGEM-T-3ABC,the exceptional primers were designed and amplified by RT-PCR to get the non-structure protein 3AB and 3A genes.They were directed to be cloned into prokaryotic expression carrier pET-28a and pET-41a respectively.The recombinant plasmids were transformed into hose BL21,and the target proteins were induced by IPTG in BL21.The expressed proteins were examined by SDS-PAGE and Western blotting.The result showed that recombinant proteins were expressed in E.coli successfully.