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HPLC-ELSD Determination of 1-Deoxynojirimycin in Mulberry Leaves

Jiang Yong-tao

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Abstract

Objective:To develop a method to determine the contents of 1-deoxynojirimycin(1-DNJ) in Morus alba leaves. Method: With the use of Evaporative Light Scattering Detector(ELSD), an SUPELCOSIL LC-NH_2 (25 cm×4. 6 mm,5 μm) column and acetonitrile-water(80: 20) as mobile phase, the flow rate was 0. 8 mL·min~(-1). Results: The calibration curves of 1-deoxynojirimycin showed good linearity in the range of 2. 28-13. 68 μg,the average recoveries was 96. 1% (n= 6),with RSD of 2. 2%. Conclusion: It was proved that the method with HPLC-ELSD was simple, the results were reliable and accurate, it can be used for the quality control of Alkaloids in Morus alba leaves.

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What this paper is about

Objective:To develop a method to determine the contents of 1-deoxynojirimycin(1-DNJ) in Morus alba leaves. Method: With the use of Evaporative Light Scattering Detector(ELSD), an SUPELCOSIL LC-NH_2 (25 cm×4. 6 mm,5 μm) column and acetonitrile-water(80: 20) as mobile phase, the flow rate was 0. 8 mL·min~(-1). Results: The calibration curves of 1-deoxynojirimycin showed good linearity in the range of 2. 28-13. 68 μg,the average recoveries was 96. 1% (n= 6),with RSD of 2. 2%. Conclusion: It was proved that the method with HPLC-ELSD was simple, the results were reliable and accurate, it can be used for the quality control of Alkaloids in Morus alba leaves.

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Available abstract

Objective:To develop a method to determine the contents of 1-deoxynojirimycin(1-DNJ) in Morus alba leaves. Method: With the use of Evaporative Light Scattering Detector(ELSD), an SUPELCOSIL LC-NH_2 (25 cm×4. 6 mm,5 μm) column and acetonitrile-water(80: 20) as mobile phase, the flow rate was 0. 8 mL·min~(-1). Results: The calibration curves of 1-deoxynojirimycin showed good linearity in the range of 2. 28-13. 68 μg,the average recoveries was 96. 1% (n= 6),with RSD of 2. 2%. Conclusion: It was proved that the method with HPLC-ELSD was simple, the results were reliable and accurate, it can be used for the quality control of Alkaloids in Morus alba leaves.

Key concepts: Chromatography detector, Chemistry, Chromatography, Calibration curve, High-performance liquid chromatography, Acetonitrile, Detection limit

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