2012•Journal of Southeast UniversityRequires access
Expression and localization of MTA1 in the gastric cancer cell lines and tissues and clinical significance
Hongmei Zhao
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Abstract
Objective:To explore the expression and the localization of MTA1 in the gastric cancer cell lines,and research the expression of MTA1 in the gastric cancer tissues and clinical significance.Methods:Total protein was extracted from gastric cancer cell lines.The expression of endogenous MTA1 was proved by Western blot.The localization of endogenous MTA1 in the gastric cancer cell lines was observed by using laser scanning confocal microscopy.The clinical gastric cancer tissues were collected,which were detected the expression of MTA1.Results:Six cell lines express MTA1 in eight gastric cancer cell lines,its localization was mainly in the nucleus.The rate of MTA1 overexpression was 63.6%(11 pairs) in the gastric cancer tissues.Conclusion:There are expression of MTA1 in the gastric cancer cell lines,which was expressed in nucleus.MTA1 was expressed in the gastric cancer tissues,it provide theoretical basis for clinical diagnosis.