Clinical value of glycosyl-phosphatidyl inositol anchored protein detection on diagnosis of paroxysmal nocturnal hemoglobinuria

Objective To investigate the expression of glycosyl-phosphatidyl inositol(GPI) anchored protein on peripheral blood cells from patients with paroxysmal nocturnal hemoglobinuria (PNH) and provide a basis for diagnosis and differential diagnosis of PNH.Methods The percentages of CD55,CD59,CD16 and CD14 expression on the peripheral blood cells from 32 patients with PNH were determined by flow cytometry. Results The expression levels of CD55 and CD59 were decreased significantly on the erythrocytes and granulocytes from patients with PNH compared with control group (P0.001),the expression levels of CD55 and CD59 on granulocytes were lower than those on erythrocytes (P0.05).The expression levels of CD55 and CD59 on the thrombocytes of PNH patients were lower than those in control group(P0.001).The expression levels of CD55 and CD59 on the lymphocytes from 27 PNH patients were lower than those on granulocytes and erythrocytes,the deficiency rate was 30%.The percentage of CD16 expression varied greatly from 34% to 83% on the granulocytes,there was significant difference compared with control group (P0.001). The deficiency of CD14 clones was detected on the monocytes from PNH patients.Conclusion The diagnosis reliability of patients with PNH is increased by the assay of CD55 and CD59 on all kinds of cells of peripheral blood.The detection of CD16 and CD14 is also an optimal and helpful index to diagnose PNH.