Effect of vaproate sodium on Bax and Bcl-2 expression of hippocampus in seizures rats induced by PTZ

Objective To explore the effect of vaproate sodium (VP) on Bcl-2 and Bax expression of hippocampus in seizures rats induced by pentylenetetrazole(PTZ).Methods Twenty-four adult Wistar rats were randomly divided into normal control (NC) group, PTZ group and VAP group. A dose of PTZ [35 mg/(kg·day)] was intraperitoneally injected into the rats of PTZ group and VAP group until the kindling criterion was reached. After kindling, the rats of VAP group were administered intraperitoneally with VAP[15 mg/(kg·day)];the rats of PTZ group were administered intraperitoneally with normal saline. After 30 minutes, seizures were induced by administering PTZ intraperitoneally. The influence of VAP on Bcl-2 and Bax immmunoreactivity on hippocampus neurons was studied by immunohistochemistry method.Results Bax positive cells in hippocampus in PTZ group were more than in VAP group and NC group(all P 0.01). Furthermore, Bax positive cells in VAP group were more than in NC group( P 0.05). Bcl-2 positive cells in hippocampus in PTZ group were more than in NC group( P 0.05), and these cells in VAP group were more than in PTZ group and NC group(all P 0.01).Conclusion Vaproate sodium can resist the apoptosis of hippocampus neurons in seizures rats by increased expression of Bcl-2 and decreased expression of Bax.