Study on enzymatic browning and browning physiology in storage of fresh-cut lotus root

The test discusses the optimal experimental conditions determining polyphenol oxidase(PPO) activity,effects of inhibitors on polyphenol oxidase and study on the related index changes of fresh-cut lotus root during storage using the fresh-cut lotus root as the research object.The results showed that when using catechol as substratethe,the most suitable wave length is 410 nm,pH value is 7.0,the temperature is 35 ℃,concentration of substrate 0.09 mol/L and the factor browning inhibition comboination is that the concentration of the Vc is 0.20%,the concentration of the sodium sulfite is 0.10%,the concentration of the citric acid is 0.20%,the concentration of the EDTA is 0.25%.During the storage,the degree of browning increased,moisture content,solute protein content and PPO activity rose firstly and then decreased.