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2010•Bulletin of Science and TechnologyRequires access

Cloning and Sequence Analysis of 18S rRNA Gene of Nemertean Form Zhejiang Coast

Wang Mengqia

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Abstract

In order to determine the taxonomic status of Dendrorhynchus zhejiangensis from Zhejiang coast,a pair of primers were designed and synthedized according to sequence of the 18S rDNA gene of Nemertineans in GenBank.The segment of this Nemertinean’s 18S rRNA gene of Nemertinean was amplified and sequenced by the polymerase chain reaction(PCR).Meanwhile,this sequence were registered in GenBank and aligned with other nemertean 18S rDNA genes available from GenkBank to analyse their homologous similarities.Determined its taxonomic status using the Phylogenetic Tree and the morphology results.The results indicated that,the species found belongs to the family Lineidae,Order Heteronemertea.

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What this paper is about

In order to determine the taxonomic status of Dendrorhynchus zhejiangensis from Zhejiang coast,a pair of primers were designed and synthedized according to sequence of the 18S rDNA gene of Nemertineans in GenBank.The segment of this Nemertinean’s 18S rRNA gene of Nemertinean was amplified and sequenced by the polymerase chain reaction(PCR).Meanwhile,this sequence were registered in GenBank and aligned with other nemertean 18S rDNA genes available from GenkBank to analyse their homologous similarities.Determined its taxonomic status using the Phylogenetic Tree and the morphology results.The results indicated that,the species found belongs to the family Lineidae,Order Heteronemertea.

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Available abstract

In order to determine the taxonomic status of Dendrorhynchus zhejiangensis from Zhejiang coast,a pair of primers were designed and synthedized according to sequence of the 18S rDNA gene of Nemertineans in GenBank.The segment of this Nemertinean’s 18S rRNA gene of Nemertinean was amplified and sequenced by the polymerase chain reaction(PCR).Meanwhile,this sequence were registered in GenBank and aligned with other nemertean 18S rDNA genes available from GenkBank to analyse their homologous similarities.Determined its taxonomic status using the Phylogenetic Tree and the morphology results.The results indicated that,the species found belongs to the family Lineidae,Order Heteronemertea.

Key concepts: GenBank, Phylogenetic tree, 18S ribosomal RNA, Biology, Gene, Sequence analysis, Cloning (programming), Sequence (biology)

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Cloning and Sequence Analysis of 18S rRNA Gene of Nemertean Form Zhejiang Coast — Research Paper | ScholarLens