Production of Shanxi mature vinegar with high ethyl acetate by liquid state fermentation

Shanxi mature vinegar with high ethyl acetate was produced by liquid state fermentation,in which,sorghum was used as the source of sugar,and Shanxi mature vinegar Daqu and two strains 10-JN-1 and 3-C-2 isolated from Shanxi mature vinegar grains were used as the starter.The fermentation conditions in the alcohol fermentation and acetic acid fermentation were optimized through orthogonal experiment.When alcohol fermentation was finished,the amounts of ethyl acetate and alcohol were increased to 37mg/100ml and 4.1g/100ml when 16% sugar was added,respectively.After the acetic acid fermentation was finished,the amount of acetic acid reached 5g/100ml.The properties of product can meet the national standard of GB2719-1996.Compared with traditional production,the production time was shorten,and the yield of ethyl acetate was double.