Ultrastructural changes of blood cells induced by various doses of ~(60)Co irradiation

Objective To understand the understand the ultrastructural changes of blood cells irradiated by different doses of 60 Co,including red blood cells,leukocyte(neutrophils,basophils,eosinophils,lymphocytes and monocytes)and platelet.Method Using transmission electron microscopy to observe the blood cells morphology.Result After irradiation,some neutrophils and lymphocytes showed ultrastructure changes of abnormal cellular structure and apoptosis,accounting for 7%～24% of total nucleated cells.The percentages of abnormal cells and apoptosis incereased with incerasing irradiation doses.No abnormalities were observed in basophils,eosinophils,monocytes,platelets,and red blood cells.Conclusion 15～35Gy 60 Co irradiation influenced the ultrastructure of neutrophils and lymphocyte,but the other cells were not influenced.