Treatment of Bone Defect Bone Allograft with Bone Marrow Stem Cell in Animal Experiment

【Objective】 To study the feasibility and result of transplanting bone allograft with bone marrow stem cell in treatment of bone defect in animal experiment.【Methods】 Fifteen New Zealand white rabbitswere chosen for the 1 cm bone defect model of both radiuses.Randomly,one radial side was chosen for the model,and the other side was for the control one.The bone defect of experimental side was filled with surface-demineralized bone allograft and bone marrow stem cell cultured in vitro.The control side was filled with bone allograft only.After 12 weeks,all the animals were observed by X ray,biomechanics,and histology.The results were analyzed and compared.【Results】 After 12 weeks,the X ray density,the torsional moment and angle in failure load test and the score of callus in histological grading were all improved in the experimental side,compared with the control side.There was not statistically significant difference in both mature degree of bone union and growth degree of bone marrow.【Conclusion】 Bone marrow stem cell can enhance the osteogenesis of bone allograft after transplantation,which increases the bone formation without affecting the development of bone tissue.