Optimization of Extraction and Purification Technology for Dahuang Tongqi Oral Liquid

Objective:To optimize extraction and purification technology for Dahuang Tongqi oral liquid.Method:With volume of volatile oil as index,orthogonal test was used to optimize extraction technology of volatile oil from Aucklandia lappa,Citrus sinensis and so on with soaking time,extraction times,the amount of water as factors;With yield of water extract,total content of rhein,emodin and chrysophanol as indexes,orthogonal test was used to investigate factors of water extraction process for Dahuang Tongqi oral liquid,such as extraction time,extraction times and the amount of water;With yield of dry extract,total extraction ratio of rhein,emodin and chrysophanol as indexes,orthogonal test was used to investigate factors of ethanol precipitation process for Dahuang Tongqi oral liquid,such as relative density of liquid,the concentration of ethanol and alcohol precipitation time.Result:Optimized extraction technology of volatile oil was:distilling 2 h with 4 times the amount of water;optimum water extraction technology was:boiling 2 h with 8 times the amount of water,2 h per time;optimized alcohol precipitation process was:concetrated to relative density of 1.20(80 ℃),95% ethanol was added to liquid contained ethanol of 50%,alcohol precipitation time 24 h.Conclusion:Optimized extraction and purification technology for Dahuang Tongqi oral liquid was reasonable and feasible,it was suitable for requirements of industrial production.