Ilexgenin A induced Apoptosis in Cervical Cancer HeLa cells and Its Mechanisms

Objective: To study the Ilexgenin A-induced apoptosis in cervical cancer HeLa cells and Its mechanisms. Methods: DAPI was adopted for nucleus stain to observe the morphoiogy change during apoptosis; Apo-ONEHomogeneous protease-3 /7 kit was used for measure the activity of Caspase-3 /7; and the binding activity of nuclear extracts of cervical cancer HeLa cells NF-κB p65 with DNA was determined to investigate the activation level of NF-κB. Results: DAPI assay showed that Karyopyknosis and chromatin condensation occurred and apoptotic bodies appeared 24 h after Ilexgenin A( IA) induction. IA enhanced the activity of Caspase-3 /7 at the manner of dose-dependence. IA assumed remarkable inhibitory effect on the activation of subunit p65 of NF-κB,and this inhibitory effect was found dose-independent to some extent. Conclusion: IA could remarkably induce the apoptosis in cervical cancer HeLa cells at the manner of concentration dependence.