STUDY ON GENOTYPING OF MYCOBACTERIUM TUBERCULOSIS STRAINS ISOLATED IN HENAN WITH MYCOBACTERIAL INTERSPERSED REPETITIVE UNITS AND MULTIPLE LOCUS VARIABLE NUMBER OF TANDEM REPEATS

[Objective] To investigate the genotyping and distribution pattern of the variable number tandem repeats(VNTR)of Mycobacterium tuberculosis clinical isolates in Henan province with Multi-locus VNTR analysis preliminarily.[Methods]M.tuberculosis clinical isolates selected from Henan province randomly were cultured with Lowenstein-Jensen medium.The genome DNA was extracted from the bacteria were collected.The polymorphism of VNTR locus was tested in the DNA samples with PCR respectively.The clustering of genotype was analyzed with BioNumerics(Version5.0).Chi-Square test was used for statistical analysis.[Results]12 MIRUs loci of 317 M.tuberculosis clinical isolates were analyzed respectively.The results showed that there were obvious polymorphisms of VNTRs.The clustering of genotype showed that these strains could be categorized into gene clusters(Ⅰ、Ⅱ、Ⅲ、Ⅳ和Ⅴ)and 292 genotypes.4.7% were clusterⅠincluding 15genotypes,67.8% were cluster Ⅱ including 198 genotypes,22.7% were clusterⅢ including 64 genotypes,1.8% were cluster Ⅳ including 4 genotypes,and 3.5% were cluster Ⅴ including 11 genotypes.[Conclusion]The results showed that there were obvious polymorphisms of MIRUs of M.tuberculosis clinical strains preliminarily.There were at least 5 clusters of MIRU genotype and clusterⅡwas the predominant prevalent strain in Henan province.