Changes of Calcium/Calmodulin-dependent Protein Kinase-II in Myocardium Nucleus and Sarcoplasmic Reticulum in Heart Failure Rabbits

Objective:To explore the changes of protein expression and activity of calcium/calmodulin-dependent protein kinase-Ⅱ(CaMK-Ⅱ) in myocardium nucleus and sarcoplasmic reticulum in experimental rabbits with heart failure(HF).Methods:A total of 16 rabbits were divided into 2 groups:Sham group and HF group,the HF model was established by volume overload plus pressure overload.n=8 in each group and all animals were treated for 7 weeks.Left ventricular structure,hemodynamic parameters and protein expression and activity of CaMK-Ⅱ in myocardium nucleus and sarcoplasmic reticulum were examined and compared between 2 groups.Results:Compared with Sham group,HF group presented increased left ventricular mass index(LVMI)(1.32 ± 0.06) g/kg vs(3.61 ± 0.09) g/kg,LVEDP(-1.50 ± 0.50) mmHg vs(23.00 ± 2.37) mmHg,all P0.05;while decreased left ventricular shorten fraction(37.83 ± 3.58)%vs(17.38 ± 3.13)%and LVEF(71.92 ± 4.56)%vs(38.50 ± 6.07)%,all P0.05.The protein expression and activity of CaMK-Ⅱ were both higher in HF group than Sham group,all P0.05.Conclusion:Increased protein expression and activity of CaMK-Ⅱ in myocardium nucleus and sarcoplasmic reticulum might be one of the mechanisms for HF occurrence in experimental rabbits.