2009•Guangdong nongye kexueOpen access

Stable Expression of Bovine Prion Protein in CHO/dhfr-cells

Ying Hong

Abstract

The target genes from T-vector were connected with dhfr co-amplified gene of eukaryotic vector and constructed pCI-PrP102.Purified recombinant plasmids were transferred into CHO/dhfr-cell through liposome,and obtained the stable expression of the cell strains after MTX screening,and indirect immunity fluorescence analysis detected the expression of the goal protein.

About this research paper

What this paper is about

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Key concepts: Chinese hamster ovary cell, Recombinant DNA, Molecular biology, Plasmid, Expression vector, Gene, Biology, Vector (molecular biology)