Effect of Apigenin on Proliferation and Apoptosis in Human NSCLC SPC-A1 Cell Line

Objective: To investigate the effect of apigenin on the growth inhibition and apoptosis in human lung cancer cell line SPC-A1. Method: Lung cancer cell line SPC-A1 were cultured in vitro routinely with different concentration( 5-80 μmol·L- 1). Cell proliferation was detected by MTT. Hoechst 33258 was used to detect the morphological change of apoptotic cells. Stained by Annexin V and propidium iodide( PI),the cells were observed by flow cytometry for apoptosis. Result: The proliferation of human lung cancer cell line SPC-A1 could be inhibited and apoptosis could be induced by apigenin. The inhibition rate and apoptosis rate were different with the different concentrations. The 50% inhibitory concentration( IC 50) at the 24,48,72 h was 319.02,37.23, 18. 59 μmol·L- 1respectively. Apoptotic body could be observed by Hoechst 33258 dying. Flow cytometry showed that apigenin could induce SPC-A1 cell apoptosis distinctly at different levels. Conclusion: Apigenin could inhibit the proliferation and induce apoptosis of human non-small cell lung cancer SPC-A1.