Enhanced bio-succinic acid synthesis based on glucose-fed with pH-constant

A strategy based on pH-constant is established by investigating the ratio of glucose to Na2CO3 consumption during the succinic acid production stage.This method can maintain the glucose concentration at a constant level.Compared with the batch fermentation,it is favourable for succinic acid accumulation when pH-constant fermentation is adopted and glucose concentration is maintained at a low level.In the case of the glucose fed-batch fermentation with pH-constant and controlling the glucose mass concentration at 10 g/L,the succinic acid titer and productivity reaches 57.6 g/L and 1.15 g/(L·h),respectively.