The Construction of Different Promoter Expression Vector and its Activity Analysis in vitro

The envelope(env)gene of reticuloendotheliosis virus(REV)as a report gene,could be used for quantitative detection of different promoters transcription activity in vitro,and provide the basis for choosing suitable promoter of living-vector vaccine of Marek's disease virus(MDV).We constructed six recombinant plasmids,containing different promoter and expressing envgene,transfected into chicken embryo fibroblast,detected different promoter transcriptional and expressional level by indirect immunofluorescence test and fluorescent quantitative PCR.The results showed that different promoters had different activity and all expressed envgene in vitro.Comparing to the other promoters,we found the own promoter activity of MDV was weak,the pec was the strongest in vitro.