Tissue culture and rapid propagation of Lysionotus aeschynanthoides

Tissue culture and rapid propagation technique of Lysionotus aeschynanthoides were studied. The results showed that the axillary buds of Lysionotus aeschynanthoides could directly germinate in MS+6-BA 1.0 mg·L-1+IBA 0.1 mg·L-1and the germination rate was 75%. The primary culture medium for bud induction from stem segments was MS+6-BA 1.0 mg·L-1+IBA 0.1 mg·L-1. The subculture medium for bud multiplication and seedlings growing was 1/2MS+NAA 0.2 mg·L-1,and proliferation coefficient was 6.0/60 d. Callus could be inducted through the pedicels explants in subculture but could not develop further. The optimal rooting medium was 1/2 MS+BA 0.2 mg·L-1+NAA 0.8 mg·L-1+AC 0.1%+5% banana juice,and rooting rate was 100%. The rooting plantlets were transplanted in greenhouse that simulated the natural growing environment of L. aeschynanthoids,the survival rate was more than 95%.