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2011•Guangdong nongye kexueOpen access

Cloning and sequence analysis of Cu/Zn superoxide dismutase gene 2(MrSOD2) cDNA from Myrica rubra

Jing Lin

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Abstract

A PCR-based homologous cloning strategy was used to identify superoxide dismutase(SOD) genes from the leaves of Myrica rubra.Results of sequence analysis indicated that a 233 bp cDNA with a conserved regions encoding a 77 amino acids actin ortholog was successfully cloned and characterized.The SOD contained the signature sequence of Cu/Zn-SOD gene family.Multiple alignment analysis of amino acid sequences of the putative SOD conserved regions in Myrica rubra was more than 86% homologous with the best scoring results from similarity searches of sequence databanks.This gene was named as MrSOD2(Genbank accession No.AB 661320).

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What this paper is about

A PCR-based homologous cloning strategy was used to identify superoxide dismutase(SOD) genes from the leaves of Myrica rubra.Results of sequence analysis indicated that a 233 bp cDNA with a conserved regions encoding a 77 amino acids actin ortholog was successfully cloned and characterized.The SOD contained the signature sequence of Cu/Zn-SOD gene family.Multiple alignment analysis of amino acid sequences of the putative SOD conserved regions in Myrica rubra was more than 86% homologous with the best scoring results from similarity searches of sequence databanks.This gene was named as MrSOD2(Genbank accession No.AB 661320).

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Available abstract

A PCR-based homologous cloning strategy was used to identify superoxide dismutase(SOD) genes from the leaves of Myrica rubra.Results of sequence analysis indicated that a 233 bp cDNA with a conserved regions encoding a 77 amino acids actin ortholog was successfully cloned and characterized.The SOD contained the signature sequence of Cu/Zn-SOD gene family.Multiple alignment analysis of amino acid sequences of the putative SOD conserved regions in Myrica rubra was more than 86% homologous with the best scoring results from similarity searches of sequence databanks.This gene was named as MrSOD2(Genbank accession No.AB 661320).

Key concepts: Myrica rubra, GenBank, Gene, Cloning (programming), Sequence analysis, Complementary DNA, Accession number (library science), Biology

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Cloning and sequence analysis of Cu/Zn superoxide dismutase gene 2(MrSOD2) cDNA from Myrica rubra — Research Paper | ScholarLens