Effect of HSP90 Peptide Complex on Expression of CD86,CD11c and IL-12p70 in Dendritic Cells

Objective To study the effect of HSP90 peptide complex on dendritic cells(DC),and to explore the antitumor effect of dendritic cell vaccine modified by HSP90 peptide complex. Methods IL-12p70 production was detected by ELISA and the expression of CD86 and CD11c was analyzed by flow cytometry before and after DC stimulating by HSP90 peptide complex.C57BL/6 mouse models of Lewis lung cancer were established to observe the inhibition of tumor by different groups of dendritic cell vaccine. Results After DC stimulating by HSP90 peptide complex,the expression of CD86 and CD11c(94.49±1.49 and 81.87±1.64%),the secretion of IL-12p70(97.11±5.26 pg/ml)were higher than those in tumor coarse extract stimulating DC group and non-stimulating blank DC group(P0.05).DC vaccine modified by HSP90 peptide complex could significantly inhibit tumor growth,with the inhibited rate of 57.56% and the apoptotic rate of 26.35%,which were both significantly higher than those of control group(P0.05). Conclusions HSP90 peptide complex can promote dendritic cell maturation,and dendritic cell vaccine modified by HSP90 peptide complex can inhibit tumor growth,which may be correlated with its induction of tumor cell apoptosis.