2007•Zhongguo Yike Daxue xuebaoRequires access
Effect of neural stem cell transplantation on glial cell line-derived neurotrophic factor after spinal cord injury in rats
Yanfeng Wang
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Abstract
Objective:To observe the effect of neural stem cell(NSC)transplantation on glial cell line-derived neurotrophic factor(GDNF)after spinal cord injury(SCI)in rats,and to investigate the mechanism of repairing SCI by NSC transplantation.Methods:NSCs derived from the hippocampus of neonatal rats were cultured and identified by immunocytochemistry.Sixty adult Wistar rats were randomly divided into 3 groups.The rat model of SCI was established in groups A and B,and the rats were injected with NSCs in group A and DMEM solution in group B 7 days after SCI,respectively.The rats in group C were normal controls.The expression of GDNF at the injured site and in the adjacent areas was detected by reverse transcription polymerase chain reaction(RT-PCR)and immunohistochemistry.Results:The results of RT-PCR showed that the expression of GDNF mRNA in group A was significantly higher than that in group B on days 1,3,and 5 of NSC transplantation.The results of immunohistochemistry indicated that the expression of GDNF in group A was significantly higher than that in group B on days 7,14,and 28 of NSC transplantation.Conclusion:The transplantation of NSC could up-regulate the expression of GDNF,which may be one of the mechanisms of repairing SCI by NSC transplantation.