Effect of activated charcoal on acute tetramethylene disulphotetramine poisoning in rabbits

Objective To study the effect of activated charcoal by oral administration on acute tetramethylene disulphotetramine (tetramine) poisoning in rabbits.Methods 33 rabbits were randomly divided into 6 groups:group A,low dose infection withont treatment;group B,low dose infection with early treatment;group C,low dose with delayed treatment;group D,lethal dose infection without treatment;group E,lethal dose with treatment;group F,dimethyl sulfoxide group.The blood and stool in rabbits were collected at different time points after relevant management.The concentrations of tetramine in plasma and in stool were determined by GC/NPD,the analysis of toxicokinetics was carried out,and the symptoms of poisoning in rabbits were recorded.Results The concentration of tetramine in plasma and all parameters of toxicokinetics were significantly different among three groups with low dose infection.Compared with group A,T ke1/2 in group B and group C were reduced by 50%,but clearance rate was increased 3-fold and 2-fold,respectively,and area under the curve(AUC) were reduced by 71.2% and 54.1%,respectively.In the first 3 days,the concentration of tetramine in stool in group B and group C were obviously higher than in group A.The mortality,quadriplegia and convulsion in group D were obviously higher than in group E.Conclusion Oral administration of activated charcoal can reduce the absorption of tetramine in rabbits and increase the excretion of tetramine.Thus symptoms of poisoning are lessened,and the mortality is reduced.