Multiplex RT-PCR for Detection of Classical Swine Fever Virus and Japanese Encephalitis Virus

Classical swine fever virus(CSFV) and Japanese encephalitis virus(JEV) are the major causes of some serious reproductive diseases and co-infection is common.Therefore rapid and reliable diagnosis is crucial for effective prevention and treatment.In this study,a multiplex RT-PCR was developed using two-pairs primers designed according to conserved sequences in E2 fragments of CSFV and that in E fragments of JEV.PCR products of 508bp and 1015bp were obtained for respective viruses.Ten organ samples(lung,lumph,liver,spleen,etal) were respectively detected by the multiplex RT-PCR,out of which four were CSFV positive,three were JEV positive,the control samples were negative.The results showed that the multiplex RT-PCR of CSFV and JEV was a rapidly specific and sensitive diagnostic method.