2007•Heilongjiang Bayi Nongken Daxue xuebaoRequires access

Study on Some Characteristics of Protease from Alkaliphile S9

QI Gui-n

Abstract

Enzyme characteristics of protease from alkaliphile S9 were investigatedin this study. The protease was purified by means of ammonium sulfate precipitation and dialysis. Results showed that the optimal temperature and pH for hydrolysis of casein were 60 ℃ and 9.0 respectively. And the protease was stable at 40 ℃ and pH 8.0～10.0. Different metal ions showed different effects on the protease activity. K+, Ca2+, Mn2+ and Mg2+ could increase the protease activity, whereas Zn2+, Cu2+, Pb2+, Fe3+ and Hg2+ could cause inhibition.

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Key concepts: Protease, Casein, Chemistry, Enzyme, Hydrolysis, Ammonium sulfate, Ammonium sulfate precipitation, Ammonium