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Construction of Fab fragment phage antibody library and selection of clones expressing anti-human β_1-AR antibody

Zuo Run

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Abstract

Objective:To construct a human Fab phage antibody library and to obtain some recombinant clones which can express Fab fragment antibody against β 1-adrenergic receptor.Methods:Fd heavy chain gene and κ?λ light chains gene of IgG obtained by RT-PCR from peripheral lymphocytes of DCM patients whose anti β 1-AR antibodies are present were cloned into pComb3 vector and the human Fab phage antibody library was constructed.The library was panned by phage display technology with β 1-ARECⅡ as antigen.Results:A human Fab phage antibody library with 1.4×10 6 capability was constructed successfully,a positive clone against human β 1-AR was screened from the phage antibody library.Conclusion:The recombinant clones which express Fab antibody against β 1-AR can be obtained by phage display technology.

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What this paper is about

Objective:To construct a human Fab phage antibody library and to obtain some recombinant clones which can express Fab fragment antibody against β 1-adrenergic receptor.Methods:Fd heavy chain gene and κ?λ light chains gene of IgG obtained by RT-PCR from peripheral lymphocytes of DCM patients whose anti β 1-AR antibodies are present were cloned into pComb3 vector and the human Fab phage antibody library was constructed.The library was panned by phage display technology with β 1-ARECⅡ as antigen.Results:A human Fab phage antibody library with 1.4×10 6 capability was constructed successfully,a positive clone against human β 1-AR was screened from the phage antibody library.Conclusion:The recombinant clones which express Fab antibody against β 1-AR can be obtained by phage display technology.

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Available abstract

Objective:To construct a human Fab phage antibody library and to obtain some recombinant clones which can express Fab fragment antibody against β 1-adrenergic receptor.Methods:Fd heavy chain gene and κ?λ light chains gene of IgG obtained by RT-PCR from peripheral lymphocytes of DCM patients whose anti β 1-AR antibodies are present were cloned into pComb3 vector and the human Fab phage antibody library was constructed.The library was panned by phage display technology with β 1-ARECⅡ as antigen.Results:A human Fab phage antibody library with 1.4×10 6 capability was constructed successfully,a positive clone against human β 1-AR was screened from the phage antibody library.Conclusion:The recombinant clones which express Fab antibody against β 1-AR can be obtained by phage display technology.

Key concepts: Phage display, Antibody, Recombinant DNA, Molecular biology, Phagemid, clone (Java method), Immunoglobulin light chain, Immunoglobulin Fab Fragments

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Construction of Fab fragment phage antibody library and selection of clones expressing anti-human β_1-AR antibody — Research Paper | ScholarLens