2005•Unpublished venueRequires access
Comparison of lymphocyte subsets in cord blood and adult bone marrow
Xu Li
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Abstract
To study the mechanism of delayed immune reconstitution, graft,versus,host disease(GVHD) and graft,versus,leukemia(GVL) effect after cord blood transplantation, the differences of T lymphocyte subsets, B lymphocyte subsets and NK cells subsets and the difference of the serum levels of soluble interleukin,2 receptor (sIL,2R) and IgG in cord blood and bone marrow were compared. The amounts of CD3+, CD3+CD5+, CD3+CD45RA+,CD3+CD45RO+, CD3+CD25+, CD19+, CD19+CD10+, CD19+CD40+, CD19+CD23+, CD3,CD16+CD56, and CD3,CD16+CD56+ cells in cord blood and bone marrow were measured with flow cytometry. The serum levels of sIL,2R and IgG in cord blood and bone marrow were measured by using enzyme,linked immunosorbent assay(ELISA) and rate immunonephelometry method. It was found that there was no marked changes in the amounts of CD3+ cells of cord blood and bone marrow(P0.05). The amounts of CD3+CD45RA+, CD19+, CD19+CD10+ and CD3,CD16+CD56, cells in cord blood were significantly increased compared with bone marrow(P0.05). While the amounts of CD3+CD5+, CD3+CD45RO+, CD3+CD25+, CD19+CD40+, CD19+CD23+ and CD3,CD16+CD56+ cells in cord blood were significantly decreased compared with bone marrow(P0.05). The serum levels of sIL,2R and IgG in cord blood were significantly decreased compared with bone marrow(P0.05), and the amounts of mature T lymphocyte subsets, mature B lymphocyte subsets and mature NK cells subsets in cord blood were significantly decreased compared with bone marrow. It concludes that these may be one of cause of delayed immune reconstitution lower GVHD and lower GVL effect after cord blood transplantation.