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Full-length cDNA cloning and sequence analysis of Mn/Fe superoxide dismutase gene in Evodia rutaecarpa

Shouwen Zhang

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Abstract

Objective To clone the full-length cDNA sequence of Mn/Fe superoxide dismutase(Mn/Fe-SOD) gene of Evodia rutaecarpa.Methods Four gene specific primers were designed according to the previously obtained core sequence of Mn/Fe-SOD gene fragment in E.rutaecarpa.RACE and nested PCR technologies were used to clone the full-length cDNA of Mn/Fe-SOD gene in E.rutaecarpa.Results The full-length cDNA sequence of Mn/Fe-SOD gene composed of 1 048 bp was obtained including an open reading frame(ORF) with 687 bp corresponding to 228 amino acids.Conclusion The full-length cDNA sequence of Mn/Fe-SOD gene in E.rutaecarpa is cloned and reported for the first time,which lays a foundation for researching the gene excess expression in order to increase stress resistance of E.rutaecarpa.

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What this paper is about

Objective To clone the full-length cDNA sequence of Mn/Fe superoxide dismutase(Mn/Fe-SOD) gene of Evodia rutaecarpa.Methods Four gene specific primers were designed according to the previously obtained core sequence of Mn/Fe-SOD gene fragment in E.rutaecarpa.RACE and nested PCR technologies were used to clone the full-length cDNA of Mn/Fe-SOD gene in E.rutaecarpa.Results The full-length cDNA sequence of Mn/Fe-SOD gene composed of 1 048 bp was obtained including an open reading frame(ORF) with 687 bp corresponding to 228 amino acids.Conclusion The full-length cDNA sequence of Mn/Fe-SOD gene in E.rutaecarpa is cloned and reported for the first time,which lays a foundation for researching the gene excess expression in order to increase stress resistance of E.rutaecarpa.

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Available abstract

Objective To clone the full-length cDNA sequence of Mn/Fe superoxide dismutase(Mn/Fe-SOD) gene of Evodia rutaecarpa.Methods Four gene specific primers were designed according to the previously obtained core sequence of Mn/Fe-SOD gene fragment in E.rutaecarpa.RACE and nested PCR technologies were used to clone the full-length cDNA of Mn/Fe-SOD gene in E.rutaecarpa.Results The full-length cDNA sequence of Mn/Fe-SOD gene composed of 1 048 bp was obtained including an open reading frame(ORF) with 687 bp corresponding to 228 amino acids.Conclusion The full-length cDNA sequence of Mn/Fe-SOD gene in E.rutaecarpa is cloned and reported for the first time,which lays a foundation for researching the gene excess expression in order to increase stress resistance of E.rutaecarpa.

Key concepts: Complementary DNA, Gene, Molecular biology, Open reading frame, Cloning (programming), Rapid amplification of cDNA ends, clone (Java method), Biology

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Full-length cDNA cloning and sequence analysis of Mn/Fe superoxide dismutase gene in Evodia rutaecarpa — Research Paper | ScholarLens