Effect of Simvastatin on the Monocyte Chemoattractant Protein-1 Expression Induced by Lipopolysaccharide in Human THP-1 Mononuclear Cell

【Objective】To investigate the effect of simvastatin on the monocyte chemoattractant protein-1(MCP-1) expression induced by lipopolysaccharide(LPS) in human THP-1 mononuclear cell and to elucidate the role of simvastatin in anti-inflammation and anti-atherosclerosis.【Methods】The human THP-1 mononuclear cell line was cultured and divided into five groups as control group,LPS group,and low,moderate or high dose simvastatin groups.After pre-incubated with different concentration simvastatin for 2h,the three simvastatin groups and LPS group were stimulated with LPS for 24h,and then MCP-1 expression in total cellular protein and supernatant of each group was measured by Western blot and ELISA,respectively.【Results】MCP-1 expression induced by LPS in human THP-1 mononuclear cell increased significantly,which was inhibited apparently by simvastatin in a dose-dependent manner.【Conclusion】Simvastatin could inhibit MCP-1 expression in human THP-1 mononuclear cell induced by LPS,which suggests that simvastatin has a role in anti-inflammation.