The effect of atorvastatin on oxidized low-density lipoprotein(ox-LDL)-induced expression of matrix metalloproteinase-1(MMP-1) mRNA in cultured human umbilical vein endothelial cells(HUVECs)

Objective To investigate the effects of atorvastatin in different concentrations on expressions of matrix metalloproteinase-1(MMP-1) induced by oxidized low-density lipoprotein(ox-LDL) in cultured human umbilical vein endothelial cells(HUVECs).Methods Ox-LDL(100 μg/ml) was added to HUVECs cultured in vitro,when the cells grown to confluence,to treat for 24 hours to coculture with atorvastatin in different concentrations(1～30 μmol/L).Expression of MMP-1 mRNA were determined by reverse transcription-polymerase chain reaction(RTPCR).Results Ox-LDL induced expression of HUVECs MMP-1 mRNA. Atorvastatin dose-dependently inhibited the expression of MMP-1 mRNA induced by ox-LDL(1,10 and 30 μmol/L)(P0.05).Conclusions Atorvastatin could decrease dose-dependently the expression of MMP-1 mRNA in ox-LDL-induced HUVECs and has anti-inflammatory effects,which are probably related to inhibition of MMP-1 expressions.