Effect of fluoride on the function of osteoblast bone formation

Objective To observe the effects of different concentration of NaF on osteoblasts function of bone formation and dose effect relationship.Methods Osteoblasts were obtained from the calvaria of 24 hour newborn SD rats by enzymic degestion and cultuered in the medium with 10 -7 ～5× 10 -4 mol/L NaF.The ALP activity and osterocalcin were examined by biochemical assay and radioimmunologic assay (RIA) respectively.Results Results showed that the effect of NaF on ALP activity was in a double phase manner,e.g. low concentration NaF(10 -7 ,10 -6 ,10 -5 mol/L) increased ALP activity ( P 0 05) respectively,vice versa the high concentration (10 -4 ,5×10 -4 mol/L) decreased ALP activity ( P 0 05).However,the action of NaF on osterocalcin secretion appeared different from ALP activity.All above concentrations of NaF showed the stimulated effect on the secretion of osterocalcin.The highest secret rate was up to 67 14% at 5×10 -4 mol/L NaF.( P 0 05).Conclusion Quantum satis fluoride can increase osteoblasts ALP activity and osteocalcin production, facilitate osteoblasts osteogenesis function,but the range of effective dose of fluoride is very narrow.