Comparative on content of chlorogenic acid and bacteriostasis of extracts from different parts of polyploid and diploid Honeysuckle

The content of chlorogenic acid (CHA) in flower, leaf and stem of polyploid and diploid Honeysuckle was determined, and bacteriostasis of extracts was studied. High performance liquid chromatography(HPLC) and mininal inhibitory concentration (MIC). The content of CHA in Honeysuckle flower, leaf and stem is, 7.76%, 4.61% and 0 in polyploids, 5.97%, 3.94% and 0 in diploids, respectively. The MIC of extracts from Honeysuckle flower and leaf for Escherichia coli, Microccus luteus (Schroeter)Cohn and Bacillus subtilis is, 250 μg/g and 500 μg/g, 500 μg/g and 500 μg/g, 250 μg/g and 500 μg/g in polyploids, 500 μg/g and 1000 μg/g, 500 μg/g and 500 μg/g, 500 μg/g and 1000 μg/g in diploids, respectively. The content of CHA in polyploid Honeysuckle flower, leaf is significantly improved compared with diploid Honeysuckle. The bacteriostasis of extract of polyploid Honeysuckle is superior to diploid Honeysuckle. Polyploid has a higher development value than diploid Honeysuckle.