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2013•Hubei nongye kexueRequires access

Isolation and Characterization of Resistance Gene Analogs from Wild Relative of Sweet Potato

Xinsun Yang

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Abstract

The degenerate primers designed from the conserved motifs in the NBS-LRR resistance gene analog(RGA) of various plants were used to amplify NBS-LRR RGA from wild relative of Ipomoea batatas,7 NBS-LRR RGAs were obtained according to cloning and sequencing,of which the amino acid sequence contained P-loop,Kinase-2,Kinase-3 and GLPL motif of the NBS-LRR RGA.Cluster analysis of the predicted amino acid sequences grouped the 7 RGAs of I.grandifolia and the known 10 NBS-LRR RGAs in NCBI database into two classes,between which the sequence similarity was low.It was deduced that the RGAs with high similarity belonged to one gene family.

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What this paper is about

The degenerate primers designed from the conserved motifs in the NBS-LRR resistance gene analog(RGA) of various plants were used to amplify NBS-LRR RGA from wild relative of Ipomoea batatas,7 NBS-LRR RGAs were obtained according to cloning and sequencing,of which the amino acid sequence contained P-loop,Kinase-2,Kinase-3 and GLPL motif of the NBS-LRR RGA.Cluster analysis of the predicted amino acid sequences grouped the 7 RGAs of I.grandifolia and the known 10 NBS-LRR RGAs in NCBI database into two classes,between which the sequence similarity was low.It was deduced that the RGAs with high similarity belonged to one gene family.

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Available abstract

The degenerate primers designed from the conserved motifs in the NBS-LRR resistance gene analog(RGA) of various plants were used to amplify NBS-LRR RGA from wild relative of Ipomoea batatas,7 NBS-LRR RGAs were obtained according to cloning and sequencing,of which the amino acid sequence contained P-loop,Kinase-2,Kinase-3 and GLPL motif of the NBS-LRR RGA.Cluster analysis of the predicted amino acid sequences grouped the 7 RGAs of I.grandifolia and the known 10 NBS-LRR RGAs in NCBI database into two classes,between which the sequence similarity was low.It was deduced that the RGAs with high similarity belonged to one gene family.

Key concepts: Gene, Genetics, Biology, Cloning (programming), Sequence analysis, Multiple sequence alignment, Amino acid, Peptide sequence

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Isolation and Characterization of Resistance Gene Analogs from Wild Relative of Sweet Potato — Research Paper | ScholarLens